Part:BBa_K136014:Design

AracpBAD - gfp triparts

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2091
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2030
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1865
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 665
  Illegal SapI site found at 1847
  

Design Notes

AracpBAD was digested by SpeI -PstI (insert) and cloned into the psb2k3 that contain the gfp tripart .

Source

flgA promotor comes from E. coli K12 strain MG1655. gfp tripart comes from the registry.